Description
Recombinant human KRAS (G12V) (2-186) was expressed in E.coli cells using an N-terminal His tag. The KRAS (G12V) mutant is constitutively active. The gene accession number is NM_033360. The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking (1). There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and KRAS occur in varying frequencies in different tumor types (2). Other members of the RAS superfamily may also contribute to cancer